von Willebrand’s Disease Type 3 (vWD3)



Von Willebrand Disease Type III

Turnaround: 3-5 days

US: $45.00 | UK: £40.00

Breeds: Mixed Breed, Scottish Terrier, Shetland Sheepdog, Unspecified

Description

Von Willebrand Disease (vWD) is an inherited bleeding disorder that inhibits the normal blood clotting process, causing prolonged bleeding after an injury. People with this condition often experience excessive bruising, prolonged nosebleeds, and excessive bleeding following any form of trauma, including surgery, or dental work.

The primary function of von Willebrand Factor (vWF), a blood glycoprotein, is to bind itself to other proteins. Under normal circumstances, this factor is designed to clot wounds in order to stop bleeding. This can be exemplified with the common scab. The failure of vWF function inhibits the blood coagulation process (also known as clotting) and causes excessive bleeding. This is most apparent in tissues having high blood flow or narrow vessels.

There have been three separate types of vWD have been identified in dogs (and people). Of these three types there are five different genetic mutations causing vWD disorders in dogs. Genetic tests have been developed to identify all five variants. Von Willebrand's Disease type II (VWDII) is transmitted as an autosomal recessive trait with variable penetrance and is the least serious of the three that we test for. Autosomal recessive traits are traits that can be passed from either parent and require two copies of the gene to show symptoms. Animal Genetics offers tests for vWDI, vWDII, and vWDIII.

The causal mutation for vWDI is a G>A nucleotide substitution. This means that one of the nucleotides (adenine, guanine, cytosine, and thymine) has been mistakenly replaced in the DNA code with a nucleotide that isn’t meant to be there. This low concentration of normal vWF prevents normal blood coagulation, which results in excessive bleeding. Von Willebrand's Disease type III (VWDIII) is the most serious of the three. Dogs with vWDIII do not produce any von Willebrand Factor protein in their blood. This leaves them especially vulnerable to excessive bleeding.

A different genetic mutation causes vWDIII in each of the following:

  • Type A: Scottish Terrier
  • Type B: Shetland Sheepdog
  • Type C: Kooikerhondje

Symptoms include:

  • abnormal bleeding
  • abnormal blood clotting
  • bleeding gums
  • blood in the feces
  • blood in the urine
  • easy bruising
  • excessive bleeding
  • hematochezia
  • hematoma (bruising)
  • hematuria
  • nosebleeds
  • vaginal bleeding

Because vWDII is a recessive disorder, a dog must have two copies of the mutation in order for the disease to manifest. This means that a dog can have one copy of the mutation and not experience any signs or symptoms of vWDII. This dog would be known as a carrier. The carrier can then pass on either the normal gene or the mutated gene to any offspring. If two carriers are bred, there is a 25% per puppy that they will develop symptoms of vWDII.

Possible Results

Genotype Description
n/n Clear: Dog is negative for the mutation associated with von Willebrand's Disease Type III.
n/vWD3 Carrier: Dog carries one copy of the von Willebrand's Disease Type III mutation.
vWD3/vWD3 Affected: Dog has two copies of the von Willebrand's Disease Type III mutation and will be affected. The mutation will always be passed on to every offspring.

Reference

J Thromb Haemost. 2016 May;14(5):894-905. doi: 10.1111/jth.13301. Epub 2016 Apr 5. Canine models of inherited bleeding disorders in the development of coagulation assays, novel protein replacement and gene therapies. Nichols TC1, Hough C2, Agersø H3, Ezban M3, Lillicrap D2.